I am confused now. So does genetic modification include breeding? I was talking about transgenic organisms, which falls under the umbrella of genetic modification. I looked up the definition of it but I think I misunderstood it. Then I looked at some recent slides from my genetics class and I think I did get it wrong. Breeding does seem to be a modification but everything I said is still relevant since it was pretty vague. By the way, I don't know if this will help you, but I copied the text from some slides from my genetics class (the pdf file of the slides was too big to attach. The book we use is Genetics, 3rd ed by Robert Brooker, if you have it and want to look up the figures):
Genetically Modified Plants
• Selective breeding has been used for centuries
– This has produced plants with desirable characteristics
• larger, disease resistance, high-quality food
• Genetically engineered crops have been used since
mid-1990s
– In 2006, roughly 20% of all crops were transgenic
– More than 100 million hectares planted with transgenics
A. Tumefaciens Can Be Used to Make
Transgenic Plants
• The production of transgenic plants is somewhat
easier than transgenic animals
– Plant cells are totipotent
• An entire organism can be regenerated from a somatic cell
• Agrobacterium tumefaciens is a
bacterium that naturally infects plants
causing crown gall tumor
Tumor-inducing
plasmid
Transferred DNA
Figure 19.7
Tumor-inducing
plasmid
Transferred DNA
Figure 19.7
A. tumefaciens does not infect all plant species
other methods are available
– Biolistic gene transfer (i.e., biological ballistics)
• The second most common way to produce transgenic plants
• A “DNA gun” is used to shoot DNA-coated microprojectiles into the
cells
– Microinjection
• Microscopic-sized needles are used to inject DNA into the cells
– Electroporation
• An electric current is used to create transient pores in the plasma
membrane through which DNA can enter
Transgenic Plants
• Transgenic plants can be given characteristics that
are agriculturally useful
– For example, the Monsanto Company has produced plants
highly tolerant of glyphosate
(The active ingredient in the herbicide RoundupTM)
– Compared to nontransgenics, these plants grow quite well
in the presence of glyphosate-containing herbicides
• Transgenic plants have been approved for human
consumption
– The first example was the FlavrSavr tomato (Figure 19.15)
• This transgenic plant has been given the gene that encodes an
antisense RNA
– Complementary to the mRNA for polygalactorunase (an enzyme
involved in fruit ripening)
• The antisense RNA binds to the mRNA and prevents translation
• The double stranded RNA is targeted for degradation
– RNAi-mediated silencing (refer to Chapter 15)
– This is called a “Knock-down”
– the FlavrSavr does not spoil (overripe) as quickly as
traditional tomatoes